5B30
H-Ras WT in complex with GppNHp (state 1) after structural transition by humidity control
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 91.791, 91.791, 121.417 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.360 - 1.600 |
R-factor | 0.16814 |
Rwork | 0.167 |
R-free | 0.19569 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k8y |
RMSD bond length | 0.027 |
RMSD bond angle | 2.404 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.059 | 0.823 |
Number of reflections | 26298 | |
<I/σ(I)> | 49 | 3.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | PEG400, SODIUM ACETATE, CALCIUM ACETATE, MAGNESIUM CHLORIDE |