5B2S
Crystal structure of the Streptococcus pyogenes Cas9 EQR variant in complex with sgRNA and target DNA (TGAG PAM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.016, 67.822, 187.567 |
| Unit cell angles | 90.00, 111.12, 90.00 |
Refinement procedure
| Resolution | 48.005 - 2.200 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4un3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.467 |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.10_2155: ???) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.005 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 103809 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 97.8 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 15-17% PEG 3350, 0.4 M KSCN, 0.1 M Tris-acetate |
