5B2A

Crystal structure of a mutant of OspA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	33.370, 53.658, 65.756
Unit cell angles	90.00, 100.35, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.20333
Rwork	0.201
R-free	0.25013
RMSD bond length	0.020
RMSD bond angle	2.057
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	27889
<I/σ(I)>	27.7
Completeness [%]	97.2
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	40% PEG 400, 0.1M Tris pH 9.0