5B24
The crystal structure of the nucleosome containing cyclobutane pyrimidine dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-11 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.848, 108.868, 174.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.640 - 3.600 |
| R-factor | 0.2069 |
| Rwork | 0.205 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3afa |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.746 |
| Data scaling software | HKL-2000 (0.98.701) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.10.1-2155-000) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 7.750 | 3.600 |
| Rmerge | 0.111 | 0.032 | 0.475 |
| Total number of observations | 94396 | ||
| Number of reflections | 22837 | ||
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 96.0 | 91.3 | 97.6 |
| Redundancy | 4.1 | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |
