5B1E
Crystal structure of proteinase K from Engyodontium album
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 300 |
Detector technology | CCD |
Collection date | 2014-11-25 |
Detector | MPCCD |
Wavelength(s) | 1.77 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.900, 68.900, 109.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.659 - 2.300 |
R-factor | 0.1429 |
Rwork | 0.141 |
R-free | 0.18120 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.055 |
Data scaling software | CrystFEL |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 12263 |
<I/σ(I)> | 12.4 |
Completeness [%] | 100.0 |
Redundancy | 402 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 291 | NaNO3, CaCl2, MES |