5B16
X-ray structure of DROSHA in complex with the C-terminal tail of DGCR8.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2015-02-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.254, 118.136, 122.296 |
| Unit cell angles | 90.00, 102.07, 90.00 |
Refinement procedure
| Resolution | 19.930 - 3.200 |
| Rwork | 0.267 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.250 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.059 | 0.577 |
| Number of reflections | 26854 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 100mM Citrate Bis-Tris Propane (pH 7.84), 3%(v/v) polyethylene glycol (PEG) 400, 3%(w/v) PEG 20,000, 1%(v/v) ethanol. |
