5B13
Crystal structure of phycoerythrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.500, 111.850, 112.670 |
| Unit cell angles | 90.00, 91.88, 90.00 |
Refinement procedure
| Resolution | 48.933 - 2.094 |
| R-factor | 0.1996 |
| Rwork | 0.198 |
| R-free | 0.23650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.318 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.699 | |
| Number of reflections | 135827 | |
| <I/σ(I)> | 11.21 | 2.13 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium acetate, PEG4000 |
