5AZY
Crystal structure of p24delta1 GOLD domain (Native 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-11-16 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 66.798, 77.354, 41.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.471 - 1.800 |
R-factor | 0.2354 |
Rwork | 0.233 |
R-free | 0.28050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5azx |
RMSD bond length | 0.006 |
RMSD bond angle | 0.875 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.563 | |
Number of reflections | 20574 | |
<I/σ(I)> | 56.7 | 4.6 |
Completeness [%] | 98.0 | 100 |
Redundancy | 7.1 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.8 M ammonium citrate (pH 7.0) |