5AZP
Crystal structure of a membrane protein from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-23 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.90000 |
Spacegroup name | I 4 |
Unit cell lengths | 257.515, 257.515, 81.825 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.690 |
R-factor | 0.16109 |
Rwork | 0.160 |
R-free | 0.17974 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5azo |
RMSD bond length | 0.019 |
RMSD bond angle | 1.973 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.690 |
Number of reflections | 297648 |
<I/σ(I)> | 22.9 |
Completeness [%] | 99.5 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 4000, sodium acetate, sodium formate |