5AZ0

Crystal structure of aldo-keto reductase (AKR2E5) of the silkworm, Bombyx mori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-20
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 61 2 2
Unit cell lengths	112.180, 112.180, 147.628
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.000 - 2.200
R-factor	0.15905
Rwork	0.157
R-free	0.20368
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.909
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.000
High resolution limit [Å]	2.200
Number of reflections	28491
<I/σ(I)>	35.6
Completeness [%]	99.8
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	4M ammonium acetate