5AYQ

Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of VGSC beta subunits

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	103.726, 59.282, 42.369
Unit cell angles	90.00, 105.97, 90.00

Refinement procedure

Resolution	29.838 - 1.700
R-factor	0.2047
Rwork	0.203
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.855
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MrBUMP
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.700
Number of reflections	27557
<I/σ(I)>	33.4
Completeness [%]	99.9
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30%(w/v) polyethylene glycol 1500