5AYN
Crystal structure of a bacterial homologue of iron transporter ferroportin in outward-facing state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.638, 84.629, 96.809 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.404 - 2.202 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23980 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.033 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.404 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 24182 |
| <I/σ(I)> | 10.6 |
| Completeness [%] | 99.9 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8.5 | 293 | 100 mM Tris, pH 8.5, 100 mM NaK-tartrate, 100 mM KNO3, 30% PEG550MME |
