5AYH
Structure of the entire dynein stalk region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 103.325, 103.325, 69.709 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.380 - 3.011 |
| R-factor | 0.259 |
| Rwork | 0.255 |
| R-free | 0.29510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wuq |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.592 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.076 | 0.704 |
| Number of reflections | 14221 | |
| <I/σ(I)> | 36.3 | 3.6 |
| Completeness [%] | 96.3 | 96.7 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, ammonium phosphate monobasic |
