5AY9
Crystal structure of Ruminococcus albus 4-O-beta-D-mannosyl-D-glucose phosphorylase (RaMP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-08 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.900, 88.900, 286.857 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.003 - 2.500 |
| R-factor | 0.2409 |
| Rwork | 0.239 |
| R-free | 0.28060 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.052 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.010 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 15552 |
| <I/σ(I)> | 14.7 |
| Completeness [%] | 99.3 |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium nitrate, 0.1M Bis-Tris propane buffer, 20%(w/v) PEG3350 |
