5AXM

Crystal structure of Thg1 like protein (TLP) with tRNA(Phe)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-30
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97319
Spacegroup name	I 2 2 2
Unit cell lengths	75.258, 127.582, 143.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.144 - 2.210
R-factor	0.2169
Rwork	0.215
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wbz 1ehz
RMSD bond length	0.007
RMSD bond angle	0.717
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.210	2.210
Rmerge		0.692
Number of reflections	35102
<I/σ(I)>	19.4	3.2
Completeness [%]	99.7	98.6
Redundancy	7.4	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	PEG 3350, tri-potassium citrate