5AXH
Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus, D312G mutant in complex with isomaltohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-06-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.334, 99.179, 169.254 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.920 - 2.200 |
| R-factor | 0.16245 |
| Rwork | 0.160 |
| R-free | 0.20516 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5axg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.367 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.920 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.127 | 0.798 |
| Number of reflections | 67398 | |
| <I/σ(I)> | 22.2 | 3.6 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 13.7 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 0.12 M sodium dihydrogenphosphate dihydrate, 1.68 M potassium phosphate dibasic |
