5AX0
Crystal Structure of the Cell-Free Synthesized Membrane Protein, Acetabularia Rhodopsin I, at 1.52 angstrom
Replaces: 3WT9Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-27 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.990, 101.630, 43.760 |
| Unit cell angles | 90.00, 119.17, 90.00 |
Refinement procedure
| Resolution | 38.210 - 1.521 |
| R-factor | 0.1767 |
| Rwork | 0.176 |
| R-free | 0.19630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.919 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.210 | 1.580 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Number of reflections | 44164 | |
| <I/σ(I)> | 12.19 | 1.77 |
| Completeness [%] | 99.4 | 97.93 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 293 | 18% PEG400, 0.1M Hepes/NaOH, 0.2M NaCl, 10mM MgCl2, 2.5% MPD |
