5AWP
Arthrobacter globiformis T6 isomalto-dextranase complexed with isomaltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 41 |
| Unit cell lengths | 123.708, 123.708, 84.841 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.450 - 2.000 |
| R-factor | 0.15901 |
| Rwork | 0.157 |
| R-free | 0.19593 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5awo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.353 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.175 | 0.345 |
| Number of reflections | 43201 | |
| <I/σ(I)> | 40 | 13.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 293 | PEG 8000, sodium acetate buffer, potassium dihydrogenphosphate |
