5AVP
Crystal structure of Geodermatophilus obscurus L-ribose isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-20 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.533, 123.226, 109.446 |
| Unit cell angles | 90.00, 97.38, 90.00 |
Refinement procedure
| Resolution | 27.060 - 2.900 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ww3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.060 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.266 | 0.446 |
| Number of reflections | 26574 | |
| <I/σ(I)> | 5 | 1.31 |
| Completeness [%] | 91.8 | 73 |
| Redundancy | 3.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 1.26M (NH4)2SO4, Tris, Li2SO4 |
