5AVE
The ligand binding domain of Mlp37 with serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-06-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.720, 134.220, 50.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.721 - 1.800 |
| R-factor | 0.1889 |
| Rwork | 0.188 |
| R-free | 0.21360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.008 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.721 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.097 | 0.411 |
| Number of reflections | 31377 | |
| <I/σ(I)> | 11.4 | 4.7 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1M Phosphate-citrate pH 4.2, 10% (w/v) PEG-8000 and 0.2M NaCl |
