5AV7
Crystal structure of Calsepa lectin in complex with bisected glycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-12-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.608, 52.836, 54.774 |
| Unit cell angles | 90.01, 90.03, 94.05 |
Refinement procedure
| Resolution | 54.770 - 1.850 |
| R-factor | 0.24712 |
| Rwork | 0.245 |
| R-free | 0.27877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ouw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.079 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.452 | |
| Number of reflections | 44270 | |
| <I/σ(I)> | 16.7 | 1.9 |
| Completeness [%] | 97.0 | 96 |
| Redundancy | 1.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Hepes-Na (pH 7.5), 3.0 M NaCl |
