5ARK
crystal structure of porcine RNase 4 in complex with dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-09 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.190, 91.320, 93.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.500 - 2.280 |
| R-factor | 0.22917 |
| Rwork | 0.226 |
| R-free | 0.29206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ar6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.407 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.500 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.060 | 0.470 |
| Number of reflections | 29780 | |
| <I/σ(I)> | 14.7 | 2.2 |
| Completeness [%] | 98.2 | 99.2 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M SODIUM PHOSPHATE PH 6.5, 12 % PEG 8000 |
