5AR6
crystal structure of porcine RNase 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-01 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 36.287, 38.191, 86.920 |
| Unit cell angles | 90.00, 97.68, 90.00 |
Refinement procedure
| Resolution | 43.070 - 1.250 |
| R-factor | 0.12613 |
| Rwork | 0.125 |
| R-free | 0.15272 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rnf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.448 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.070 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.060 | 0.390 |
| Number of reflections | 32743 | |
| <I/σ(I)> | 15.9 | 4.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M MORPHEUS BUFFER SYSTEM 3 PH 8.5, 0.12 M MONOSACCHARIDES, 30 % (V/V) EDO_P8K |
