5APW
Sequence MATKDD inserted between GCN4 adaptors - Structure T6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.200, 27.020, 101.040 |
| Unit cell angles | 90.00, 93.93, 90.00 |
Refinement procedure
| Resolution | 34.120 - 1.600 |
| R-factor | 0.17498 |
| Rwork | 0.173 |
| R-free | 0.20468 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.897 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.420 |
| Number of reflections | 24470 | |
| <I/σ(I)> | 13.6 | 2.33 |
| Completeness [%] | 98.2 | 96.1 |
| Redundancy | 3.04 | 2.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M CACL, 0.1 M HEPES PH 7.5, 30 %(W/V) PEG 4000 |
