5APV
Sequence IANKEDKAD inserted between GCN4 adaptors - Structure A9b grey
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.090, 34.990, 106.190 |
| Unit cell angles | 90.00, 101.71, 90.00 |
Refinement procedure
| Resolution | 38.110 - 2.000 |
| R-factor | 0.20884 |
| Rwork | 0.206 |
| R-free | 0.25271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.963 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.100 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.520 |
| Number of reflections | 32657 | |
| <I/σ(I)> | 13.9 | 2.43 |
| Completeness [%] | 92.4 | 98.9 |
| Redundancy | 3.29 | 3.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM CITRATE, 0.1 M BIS TRIS PROPANE PH 6.5, 20%(W/V) PEG 3350 |
