5APS
Sequence IENKKAD inserted between GCN4 adaptors - Structure A7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 38.240, 38.240, 87.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.510 - 1.370 |
| R-factor | 0.19749 |
| Rwork | 0.195 |
| R-free | 0.23823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.942 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.200 | 1.450 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.050 | 0.760 |
| Number of reflections | 16040 | |
| <I/σ(I)> | 18.2 | 2.52 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 6.35 | 6.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M CITRIC ACID PH 3.5, 3 M NACL |
