5APP
Actinobacillus actinomycetemcomitans OMP100 residues 133-198 fused to GCN4 adaptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-27 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.130, 35.860, 198.510 |
| Unit cell angles | 90.00, 96.02, 90.00 |
Refinement procedure
| Resolution | 32.900 - 2.300 |
| R-factor | 0.22628 |
| Rwork | 0.225 |
| R-free | 0.25407 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.061 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.900 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.450 |
| Number of reflections | 18247 | |
| <I/σ(I)> | 14 | 1.88 |
| Completeness [%] | 92.4 | 86.5 |
| Redundancy | 2.84 | 2.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRI-SODIUM CITRATE PH 5.5, 2 %(V/V) DIOXANE 15 %(W/V) PEG 10,000 |
