5AP8
Structure of the SAM-dependent rRNA:acp-transferase Tsr3 from S. solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-17 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.590, 62.486, 196.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.085 - 2.246 |
R-factor | 0.2245 |
Rwork | 0.224 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.004 |
RMSD bond angle | 0.769 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.090 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.040 | 1.410 |
Number of reflections | 29932 | |
<I/σ(I)> | 21.39 | 1.41 |
Completeness [%] | 99.6 | 98 |
Redundancy | 3.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 12.5 % MPD, 12.5 % PEG1000, 12.5 % PEG 3350, 100 MM TRIS/BICINE PH 8.5, 30 MM DIETHYLENE GLYCOL,30 MM TRIETHYLENE GLYCOL, 30 MM TETRAETHYLENE GLYCOL, 30 MM PENTAETHYLENE GLYCOL |