5AP7
Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-29 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 70.720, 111.970, 116.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.660 - 2.450 |
| R-factor | 0.1882 |
| Rwork | 0.186 |
| R-free | 0.23430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c4j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.660 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.070 | 1.460 |
| Number of reflections | 17195 | |
| <I/σ(I)> | 13.4 | 1.1 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 5.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M TRIS, PH 8.0, 0.2M MGCL2, 20% (W/ V) PEG6000 |
