5AP0
Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-04 |
| Detector | QUANTUM |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 70.930, 111.960, 113.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.120 - 2.150 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c4j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.120 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.060 | 0.700 |
| Number of reflections | 23736 | |
| <I/σ(I)> | 10.9 | 0.7 |
| Completeness [%] | 95.5 | 72.9 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% (V/V) PEG300, PH 7.5 |
