5AOZ
High resolution SeMet structure of the third cohesin from Ruminococcus flavefaciens scaffoldin protein, ScaB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-13 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 60.427, 60.427, 86.509 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.540 - 1.140 |
R-factor | 0.1196 |
Rwork | 0.118 |
R-free | 0.14243 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.016 |
RMSD bond angle | 1.750 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (AUTOSOL) |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.540 | 1.810 |
High resolution limit [Å] | 1.140 | 1.140 |
Rmerge | 0.070 | 1.330 |
Number of reflections | 58923 | |
<I/σ(I)> | 18.2 | 1.73 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 10.3 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 45 MG ML PROTEIN IN 0.1 M (NH4)2SO4, 20% PEG 4K, 15% GLYCEROL, pH 7.0 |