5AOR
Structure of MLE RNA ADP AlF4 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-23 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 154.373, 154.373, 198.194 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.820 - 2.080 |
| R-factor | 0.19196 |
| Rwork | 0.190 |
| R-free | 0.22071 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kx2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.567 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.150 | 1.500 |
| Number of reflections | 143331 | |
| <I/σ(I)> | 15.1 | 1.7 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 13.25 | 13.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20 % PEG 3350 0.2 M MAGNESIUM ACETATE |
