5AOO
X-ray structure of a human Kobuvirus: Aichi virus A (AiV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-29 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 2 3 |
Unit cell lengths | 350.816, 350.816, 350.816 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.460 - 2.100 |
R-factor | 0.3301 |
Rwork | 0.330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bev |
RMSD bond length | 0.930 |
RMSD bond angle | 1.571 |
Data reduction software | XDS |
Phasing software | GLRF |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.460 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.170 | 0.870 |
Number of reflections | 186646 | |
<I/σ(I)> | 5.5 | 1.1 |
Completeness [%] | 90.0 | 83.6 |
Redundancy | 3.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.05M CADMIUM SULFATE 0.1M HEPES PH 7.5 1.0M SODIUM ACETATE |