5AO5
Endo180 D1-4, monoclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-05 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.370, 92.370, 127.650 |
| Unit cell angles | 90.00, 100.31, 90.00 |
Refinement procedure
| Resolution | 60.158 - 2.480 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1TDQ 3m7p 1dqg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.901 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.800 | 2.540 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 50153 | |
| <I/σ(I)> | 15.4 | 2.2 |
| Completeness [%] | 97.8 | 98.4 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
