5AN4
Crystal structure of the human 8-oxoguanine glycosylase (OGG1) processed with the CrystalDirect automated mounting and cryo-cooling technology
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 |
Unit cell lengths | 106.071, 106.071, 47.287 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.520 - 1.600 |
R-factor | 0.1851 |
Rwork | 0.184 |
R-free | 0.21427 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.022 |
RMSD bond angle | 1.952 |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.000 |
High resolution limit [Å] | 1.600 |
Rmerge | 0.080 |
Number of reflections | 40235 |
Completeness [%] | 99.9 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M SODIUM CITRATE, PH=5.5, 0.2 M AMMONIUM SULFATE, 24 % PEG 3350 |