5AMB
Crystal structure of the Angiotensin-1 converting enzyme N-domain in complex with amyloid-beta 35-42
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.979, 76.950, 83.219 |
| Unit cell angles | 88.63, 64.14, 75.22 |
Refinement procedure
| Resolution | 74.510 - 1.550 |
| R-factor | 0.15866 |
| Rwork | 0.158 |
| R-free | 0.18115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nxq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.439 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.620 |
| Number of reflections | 209085 | |
| <I/σ(I)> | 7.2 | 1.1 |
| Completeness [%] | 83.3 | 42.1 |
| Redundancy | 1.6 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.06 M DIVALENT CATIONS, 0.1 M TRIS/ BICINE PH 8.5, 30 % PEG550MME/PEG20000 |
