5AM9
Crystal structure of the Angiotensin-1 converting enzyme N-domain in complex with amyloid-beta 10-16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-24 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 73.348, 101.800, 113.950 |
Unit cell angles | 85.04, 85.55, 81.88 |
Refinement procedure
Resolution | 113.270 - 1.800 |
R-factor | 0.19835 |
Rwork | 0.197 |
R-free | 0.22907 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nxq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.454 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.470 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.120 | 0.760 |
Number of reflections | 291734 | |
<I/σ(I)> | 5.3 | 1.4 |
Completeness [%] | 97.0 | 95.7 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.06 M DIVALENT CATIONS, 0.1 M TRIS/BICINE PH 8.5, 30 % PEG550MME/PEG20000 |