5AKN
THE CRYSTAL STRUCTURE OF I-DMOI Q42AK120M IN COMPLEX WITH ITS TARGET DNA NICKED IN THE non-CODING STRAND B AND IN THE PRESENCE OF 2MM MN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.100, 70.340, 107.170 |
| Unit cell angles | 90.00, 119.81, 90.00 |
Refinement procedure
| Resolution | 46.490 - 2.750 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vs7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.343 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.080 | 0.610 |
| Number of reflections | 68711 | |
| <I/σ(I)> | 13.3 | 2.5 |
| Completeness [%] | 98.1 | 96.2 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
