5AKD
MutS in complex with the N-terminal domain of MutL - crystal form 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-12 |
| Detector | DECTRIS PILATUS 2MF |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 193.020, 109.762, 275.841 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 275.840 - 7.600 |
| R-factor | 0.26591 |
| Rwork | 0.264 |
| R-free | 0.30604 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1W7A 1bkn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.441 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 8.500 |
| High resolution limit [Å] | 7.600 | 7.600 |
| Rmerge | 0.170 | 0.920 |
| Number of reflections | 11763 | |
| <I/σ(I)> | 4.3 | 1 |
| Completeness [%] | 81.3 | 82.5 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9-12% PEG8000, 100 MM TRIS PH 7.0, 200 MM MGCL2, 80-450 MM SODIUM MALONATE |
