5AKC
MutS in complex with the N-terminal domain of MutL - crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 380.630, 126.460, 243.350 |
| Unit cell angles | 90.00, 91.41, 90.00 |
Refinement procedure
| Resolution | 243.280 - 6.600 |
| R-factor | 0.26067 |
| Rwork | 0.259 |
| R-free | 0.29142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1W7A AND 1BKN |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.504 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.940 | 7.130 |
| High resolution limit [Å] | 6.600 | 6.600 |
| Rmerge | 0.210 | 0.800 |
| Number of reflections | 21305 | |
| <I/σ(I)> | 3.4 | 1.1 |
| Completeness [%] | 96.8 | 97.7 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9-12% PEG8000, 100 MM TRIS PH 7.0, 200 MM MGCL2, 80-450 MM SODIUM MALONATE |
