5AKB
MutS in complex with the N-terminal domain of MutL - crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.932, 188.544, 200.426 |
| Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
| Resolution | 199.730 - 4.710 |
| R-factor | 0.32088 |
| Rwork | 0.319 |
| R-free | 0.34918 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1W7A AND 1BKN |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.422 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.700 | 4.960 |
| High resolution limit [Å] | 4.710 | 4.710 |
| Rmerge | 0.190 | 0.800 |
| Number of reflections | 31052 | |
| <I/σ(I)> | 2.5 | 1 |
| Completeness [%] | 97.3 | 98 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9-12% PEG8000, 100 MM TRIS PH 7.0, 200 MM MGCL2, 80-450 MM SODIUM MALONATE |
