5AIT
A complex of of RNF4-RING domain, UbeV2, Ubc13-Ub (isopeptide crosslink)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 77.580, 77.580, 328.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.190 - 3.400 |
| R-factor | 0.21156 |
| Rwork | 0.208 |
| R-free | 0.28617 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.556 |
| Data reduction software | xia2 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 3.490 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.030 | 0.680 |
| Number of reflections | 14922 | |
| <I/σ(I)> | 25.7 | 1.9 |
| Completeness [%] | 88.9 | 35 |
| Redundancy | 4.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
