5AIE

Not4 ring domain in complex with Ubc4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-10
Detector	DECTRIS PILATUS 2M
Spacegroup name	H 3
Unit cell lengths	107.112, 107.112, 62.203
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.556 - 2.800
R-factor	0.2246
Rwork	0.220
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.560	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.060	0.970
Number of reflections	6541
<I/σ(I)>	29.35	2.1
Completeness [%]	99.4	95.2
Redundancy	10.3	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		10% (W/V) PEG 8000, 0.02 M OF L-NA-GLUTAMATE, 0.02 M OF ALANINE (RACEMIC), 0.02 M OF GLYCINE, 0.02 M OF LYSINE HCL (RACEMIC), 0.02 M OF SERINE (RACEMIC), 0.1 M BICINE PH 8.5 AND 20% (W/V) ETHYLENE GLYCOL AS CRYSTALLIZATION BUFFER AT ROOM TEMPERATURE.