5AHN
IMP-bound form of the D199N mutant of IMPDH from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 4 |
| Unit cell lengths | 116.551, 116.551, 58.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.857 - 1.652 |
| R-factor | 0.1622 |
| Rwork | 0.161 |
| R-free | 0.18150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dqw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.620 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.200 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.030 | 0.450 |
| Number of reflections | 46336 | |
| <I/σ(I)> | 20.1 | 2.2 |
| Completeness [%] | 98.6 | 90.9 |
| Redundancy | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PEG 4000 10%, MES 0.1 M PH 7, MGCL2 0.2 M |
