5AFP
Neuronal calcium sensor-1 (NCS-1)from Rattus norvegicus complex with rhodopsin kinase peptide from Homo sapiens
Replaces: 4UYCExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Collection date | 2014-07-12 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.690, 93.690, 55.710 |
| Unit cell angles | 90.00, 92.25, 90.00 |
Refinement procedure
| Resolution | 47.860 - 2.300 |
| R-factor | 0.22708 |
| Rwork | 0.226 |
| R-free | 0.25515 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2YOU |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.238 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.370 |
| Number of reflections | 18488 | |
| <I/σ(I)> | 15.8 | 2.2 |
| Completeness [%] | 99.2 | 94.3 |
| Redundancy | 3.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.12M ALCOHOLS (1,6-HEXANEDIOL; 1- BUTANOL;1,PROPANEDIOL(RACEMIC); 2-PROPONOL; 1, 4-BUTANEDIOL; 1,3- PROPANEDIOL); 0.1M BUFFER 2 (SODIUM HEPES; MOPS ACID PH 7.5); P550MME_P20K 30%. |
