5AFG

Structure of the Stapled Peptide Bound to Mdm2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Collection date	2014-11-13
Spacegroup name	P 41 21 2
Unit cell lengths	36.821, 36.821, 177.416
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.350 - 1.900
R-factor	0.1787
Rwork	0.176
R-free	0.22616
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hg7
RMSD bond length	0.023
RMSD bond angle	1.947
Data reduction software	PROTEUM2
Data scaling software	PROTEUM2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.360	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.100	0.640
Number of reflections	10513
<I/σ(I)>	26.13	2.88
Completeness [%]	100.0	100
Redundancy	30.7	15.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		100 MM SODIUM ACETATE, 1 M AMMONIUM DI-HYDROGEN PHOSPHATE, PH 4.5