5AFB
Crystal structure of the Latrophilin3 Lectin and Olfactomedin Domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Collection date | 2014-08-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07114, 1.0716 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.430, 96.650, 101.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.910 - 2.160 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.24520 |
| Structure solution method | SIR |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.090 | 2.270 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.100 | 0.860 |
| Number of reflections | 39860 | |
| <I/σ(I)> | 10.2 | 1.7 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 3.49 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M MES BUFFER, 4M NACL, 0.6 M NON- DETERGENT SULFO-BETAINE NDSB195, PH 6.0 |
