5AF4
Structure of Lys33-linked diUb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | PILATUS |
| Spacegroup name | I 4 |
| Unit cell lengths | 113.075, 113.075, 103.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.469 - 1.850 |
| R-factor | 0.2326 |
| Rwork | 0.231 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.928 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.470 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.040 | 0.440 |
| Number of reflections | 55562 | |
| <I/σ(I)> | 11.9 | 2.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 3M AMMONIUM SULPHATE, 100 MM TRIS PH 8.0 |
