5AEX

Crystal structure of Saccharomyces cerevisiae Mep2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-30
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	103.692, 232.350, 279.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.276 - 3.200
R-factor	0.1905
Rwork	0.189
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b2j
RMSD bond length	0.012
RMSD bond angle	1.154
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	3.250
High resolution limit [Å]	3.200	3.200
Rmerge	0.090	0.920
Number of reflections	111848
<I/σ(I)>	9.6	2
Completeness [%]	99.9	100
Redundancy	4.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1 M MES PH 6.0, 0.2 M AMMONIUM PHOSPHATE DIBASIC 30% PEG400