5AEA
Crystal structure of human NCAM domain 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-20 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.850, 107.327, 41.748 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 38.436 - 1.900 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.19920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qz1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.095 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.440 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.790 |
| Number of reflections | 16311 | |
| <I/σ(I)> | 14.4 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.88 | 1.45 M TRI-SODIUM CITRATE PH 6.88 |
